Polyampholytes as Emerging Macromolecular Cryoprotectants
نویسندگان
چکیده
منابع مشابه
Instabilities of charged polyampholytes.
We consider polymers formed from a (quenched) random sequence of charged monomers of opposite signs. Such polymers, known as polyampholytes (PAs), are compact when completely neutral and expanded when highly charged. We examine the transition between the two regimes by Monte Carlo simulations, and by analogies to charged drops. We find that the overall excess charge, Q, is the main determinant ...
متن کاملExcess Charge in Polyampholytes
Polyampholytes (PAs) are polymers composed of (quenched) random sequences of positive and negatively charged monomeric groups. We show that the radius of gyration, Rg, of a PA strongly depends on its overall excess chargeQ, and is very weakly influenced by other aspects of the sequence. For Q < Qc ≈ q0 √ N , where N is the number of monomers of charge ±q0, the PA is compact, while for Q > Qc it...
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We have studied the transition in shape of two dimensional polyampholytes using Monte Carlo simulation. We observe that polymers with randomly charged monomers get into a globular shape at lower temperatures, provided that their total charge is below a critical value Q c. Collapse into globular form happens for all forms of force law, but the critical charge depends on the form of the force law...
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Significant cell damage occurs during cryopreservation resulting in a decreased number of viable and functional cells post-thawing. Recent studies have correlated the unsuccessful outcome of regenerative therapies with poor cell viability after cryopreservation. Cell damage from ice recrystallization during freeze-thawing is one cause of decreased viability after cryopreservation. We have asses...
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In connection with an investigation of the staining of intracellular antigens with ferritin-antibody conjugates (1), we required an embedding material which was extensively ionic. Although several hydrophilic embedding materials have been described (cf. reference 2), to our knowledge no satisfactory ionic ones have been reported. We sought to prepare an ionic embedding material by the bulk poly...
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ژورنال
عنوان ژورنال: Biomacromolecules
سال: 2019
ISSN: 1525-7797,1526-4602
DOI: 10.1021/acs.biomac.9b01053